Publications

2010-

  • Moogk D; Zhong S; Yu Z; Liadi I; Rittase W; Fang V; Dougherty J; Perez-Garcia A; Osman I; Zhu C; Varadarajan N; Restifo NP; Frey AB; Krogsgaard M (2016) Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets J Immunol 196: 4075-4081  PDF
  • Mahendra A; Peyron I; Thaunat O; Dollinger C; Gilardin L; Sharma M; Wootla B; Rao DN; Padiolleau-Lefevre S; Boquet D; More A; Varadarajan N; Kaveri SV; Legendre C; Lacroix-Desmazes S (2016) Generation of Catalytic Antibodies Is an Intrinsic Property of an Individual's Immune System: A Study on a Large Cohort of Renal Transplant Patients J Immunol 196: 4075-4081  PDF
  • Ramesh B; Frei CS; Cirino PC; Varadarajan N (2015) Functional enrichment by direct plasmid recovery after fluorescence activated cell sorting Biotechniques 59(3), 157-161   PDF
  • Merouane A; Villamizar NR; Liu Y; Liadi I; Romain G; Singh H; Lu J; Cooper LJN; Varadarajan N; Roysam B (2015) Automated and Quantitative Profiling of Individual Cell-Cell Interactions from High-throughput Time-lapse Imaging Microscopy in Nanowell Grids (TIMING) Bioinformatics doi: 10.1093/bioinformatics/btv355  PDF
  • Liadi I; Singh H; Romain G; Roysam B; Cooper LJN; Varadarajan N (2015) Defining potency of CAR+ T cells: Fast and Furious or Slow and Steady OncoImmunology doi: 10.1080/2162402X.2015.1051298   PDF
  • Liadi I; Singh H; Romain G; Rey-Villamizar N; Merouane A; Adolacion JR; Kebriaei P; Huls H; Qiu P; Roysam B; Cooper LJ; Varadarajan N.(2015) Individual motile CD4+ T cells can participate in efficient multi-killing through conjugation to multiple tumor cells Cancer Immunol Res doi: doi:10.1158/2326-6066.CIR-14-0195   PDF
  • Romain G; Senyukov V; Rey-Villamizar N; Merouane A; Kelton W; Liadi I; Mahendra A; Charab W; Georgiou G; Roysam B; Lee DA; Varadarajan N (2014) Antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells Blood Nov 20;124(22):3241-9. doi: 10.1182/blood-2014-04-569061   PDF
  • Ogunniyi AO; Thomas BA; Politano TJ; Varadarajan N; Landais E; Poignard P; Walker BD; Kwon DS; Love JC (2014) Profiling human antibody responses by integrated single-cell analysis. Vaccine May 19;32(24):2866-73. doi: 10.1016/j.vaccine.2014.02.020   PDF
  • Sendra VG, Lie A, Romain G, Agarwal SK, Varadarajan N (2013) Detection and isolation of auto-reactive human antibodies from primary B cells. Methods 2013 Dec 1;64(2):153-9. doi: 10.1016/j.ymeth.2013.06.018   PDF
  • Dekosky BJ, Ippolito GC, Deschner RP, Lavinder JJ, Wine Y, Rawlings BM, Varadarajan N, Giesecke C, Dorner T, Andrews SF, Wilson PC, Hunicke-Smith SP, Willson CG, Ellington AD, Georgiou G (2013) High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nat Biotech 2013 Feb;31(2):166-9. doi: 10.1038/nbt.2492   PDF
  • Liadi I, Roszik J, Romain G, Cooper LJN, Varadarajan N (2013) Quantitative High-Throughput Single-Cell Cytotoxicity Assay for T cells, J Vis Exp 2013 Feb 2;(72). doi:pii: 50058. 10.3791/50058   PDF
  • Ramesh B, Sendra VG, Cirino PC, Varadarajan N (2012) Single-cell characterization of autotransporter mediated Escherichia coli surface display of disulfide-bond containing proteins J Biol Chem Nov 9;287(46):38580-9. doi: 10.1074/jbc.M112.388199.   PDF
  • Skretas, G, Makino T, Varadarajan N, Pogson M, Georgiou G (2012). Multi-copy genes that enhance the yield of mammalian G Protein-coupled receptors in Escherichia coli. Metab Eng 2012 Sep;14(5):591-602.   PDF
  • Varadarajan N, Kwon DS, Law KM, Ogunniyi AO, Anahtar MN, Richter JM, Walker BD, Love JC (2012) Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc Natl Acad Sci U S A 109(10):3885-90.   PDF
  • Varadarajan N, Julg B, Yamanaka YJ, Chen H, Ogunniyi AO, McAndrew E, Porter LC, Piechocka-Trocha A, Hill BJ, Douek DC, Pereyra F, Walker BD, Love JC (2011) A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis J Clin Invest Nov 1;121(11):4322-31.   PDF

2005-2010

  • Varadarajan N, Pogson M, Georgiou G and Iverson BL (2009) A three-color mutli-substrate flow-cytometric assay to engineer protease specificity for nitro-tyrosine J Am Chem Soc 131(50):18186-90.   PDF
  • Varadarajan N, Cantor J, Georgiou G and Iverson BL (2009) Construction and flow-cytometric screening of targeted enzyme libraries Nat Prot 4(6):893-901.   PDF
  • Varadarajan N, Georgiou G, Iverson BL (2008) Engineered Proteases that Cleave Specifically after Sulfalted Tyrosine for the Detection of Post-Translationally Modified Peptides Angew Chem Intl Ed 47(41):7861.   PDF
  • Varadarajan N, Rodriguez S, Hwang BY, Georgiou G, Iverson BL (2008) Engineering a Family of Highly Active and Selective Endopeptidases with Programmed Substrate Specificities Nat Chem Biol 4(5):290-4.   PDF
  • Hwang BY, Varadarajan N, Li H, Rodriguez S, Iverson BL, Georgiou G (2007) Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpP J Bacteriol 189: 522-30.   PDF
  • Varadarajan N, Gam J, Olsen MJ, Georgiou G, Iverson BL (2005) Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity Proc Natl Acad Sci U S A 102:6855-60.   PDF