Leekha A et al. (2022) An intranasal nanoparticle STING agonist has broad protective immunity against respiratory viruses and variants. bioRxivPDF
Romain G et al. (2022) Multidimensional single-cell analysis identifies a role of CD2-CD58 interactions for clinical antitumor T cell responses. bioRxivPDF
Publications
Fathi M et al. (2022) Cytotoxic T lymphocytes targeting a conserved SARS-CoV-2 spike epitope are efficient serial killers. BioTechniquesPDF
Doshi A et al. (2022) Design and characterization of a salicylic acid-inducible gene expression system for Jurkat cells. Journal of BiotechPDF
Sadeghi F et al. (2021) Salicylic acid inducible nucleocytoplasmic shuttling of NPR1 fusion proteins in human cells. Biotech BioengineeringPDF
An X et al. (2021) Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2. iSciencePDF
Fathi M et al. (2021) Single-cell cloning of breast cancer cells secreting specific subsets of extracellular vesicles. CancersPDF
Joseph R et al. (2021) CD8+ T cells inhibit metastasis and CXCL4 regulates its function. British Journal of CancerPDF
Bandey I et al. (2021) Designed improvement to T-cell immunotherapy by multidimensional single cell profiling. Journal for ImmunoTherapy of CancerPDF
Yang D et al. (2021) Mesoscopic protein-rich clusters host the nucleation of mutant p53 amyloid fibrils. PNASPDF
Zheng J et al. (2021) Single cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia. iSciencePDF
Daher M et al. (2021) CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor. BloodPDF
Hou J et al. (2021) Integrating genome-wide CRISPR immune screen with multi-omic clinical data reveals distinct classes of tumor intrinsic immune regulators. Journal for ImmunoTherapy of CancerPDF
Doshi A et al. (2020) Small-molecule inducible transcriptional control in mammalian cells. Crit Rev in BiotechPDF
Rath J et al. (2020) Single-cell transcriptomics identifies multiple pathways underlying antitumor function of TCR- and CD8αß -engineered human CD4+ T cells. Science AdvancesPDF
Fousek R et al. (2020) CAR T-cells that target acute B-lineage leukemia irrespective of CD19 expression LeukemiaPDF
Mobiny A et al. (2020) Automated Classification of Apoptosis in Phase Contrast Microscopy Using Capsule Network IEEE Trans Med ImagingPDF
Ramesh P et al. (2019) Engineered Chymotrypsin for Mass Spectrometry-Based Detection of Protein Glycosylation. ACS Chem BiolPDF
Yuan P et al. (2019) Phasetime: Deep Learning Approach to Detect Nuclei in Time Lapse Phase Images J Clin Med PDF
Varadarajan N and Mohan C (2019) Elucidating the molecular circuitry of autoimmunity Nat Immunol PDF
Soundarajan R et al. (2019) Targeting the Interplay between Epithelial-to-Mesenchymal-Transition and the Immune System for Effective Immunotherapy Cancers PDF
Yankaskas C et al. (2019) A microfluidic assay for the quantification of the metastatic propensity of breast cancer specimens Nature Biomed Engineering PDF
Mahendra A et al. (2019) Beyond Autoantibodies: Biologic Roles of Human Autoreactive B Cells in Rheumatoid Arthritis Revealed by RNA-Sequencing. Arthritis Rheumatol PDF
Lu H et al. (2019) TIMING 2.0: high-throughput single-cell profiling of dynamic cell-cell interactions by time-lapse imaging microscopy in nanowell grids. Bioinformatics PDF
An X, and Varadarajan N (2018) Single-cell technologies for profiling T cells to enable monitoring of immunotherapies. Curr Opin Chemical Engg PDF
An X et al. (2017) Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells. Plos One PDF
Ritthipichai K et al. (2017) Multifaceted role of BTLA in the control of CD8+ T cell fate after antigen encounter Clin Cancer ResPDF
Varadarajan N (2017) Unmassked: Single-Cell Profiling of Immune Cell Populations in Tumors. Mol TherPDF
Lee CH et al. (2017) IgG Fc domains that bind C1q but not effector Fcg receptors delineate the importance of complement-mediated effector functions. Nat Immunol 196: 4075-4081 PDF
Moogk D et al. (2016) Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. J Immunol 196: 4075-4081 PDF
Mahendra A et al. (2016) Generation of Catalytic Antibodies Is an Intrinsic Property of an Individual’s Immune System: A Study on a Large Cohort of Renal Transplant Patients. J Immunol 196: 4075-4081. PDF
Ramesh B; Frei CS; Cirino PC; Varadarajan N (2015) Functional enrichment by direct plasmid recovery after fluorescence activated cell sorting. Biotechniques 59(3), 157-161. PDF
Merouane A et al. (2015) Automated and Quantitative Profiling of Individual Cell-Cell Interactions from High-throughput Time-lapse Imaging Microscopy in Nanowell Grids. (TIMING) Bioinformatics 31(19):3189-97. PDF
Liadi I et al. (2015) Defining potency of CAR+ T cells: Fast and Furious or Slow and Steady. OncoImmunology doi: 10.1080/2162402X.2015.1051298 PDF
Liadi I et al. (2015) Individual motile CD4+ T cells can participate in efficient multi-killing through conjugation to multiple tumor cells. Cancer Immunol Res 3(5):473-82. PDF
Romain G et al. (2014) Antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells. Blood Nov 20;124(22):3241-9. PDF
Ogunniyi AO et al. (2014) Profiling human antibody responses by integrated single-cell analysis. Vaccine May 19;32(24):2866-73. PDF
Sendra VG, Lie A, Romain G, Agarwal SK, Varadarajan N (2013) Detection and isolation of auto-reactive human antibodies from primary B cells. Methods 2013 Dec 1;64(2):153-9. PDF
Dekosky BJ et al. (2013) High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nat Biotech 2013 Feb;31(2):166-9. PDF
Liadi I et al. (2013) Quantitative High-Throughput Single-Cell Cytotoxicity Assay for T cells. J Vis Exp 2013 Feb 2;(72). PDF
Ramesh B, Sendra VG, Cirino PC, Varadarajan N (2012) Single-cell characterization of autotransporter mediated Escherichia coli surface display of disulfide-bond containing proteins. J Biol Chem Nov 9;287(46):38580-9. PDF
Skretas G et al. (2012). Multi-copy genes that enhance the yield of mammalian G Protein-coupled receptors in Escherichia coli. Metab Eng 2012 Sep;14(5):591-602. PDF
Varadarajan N et al. (2012) Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc Natl Acad Sci U S A 109(10):3885-90. PDF
Varadarajan N et al. (2011) A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis. J Clin Invest Nov 1;121(11):4322-31. PDF
Varadarajan N, Pogson M, Georgiou G and Iverson BL (2009) Proteases That Can Distinguish among Different Post-translational Forms of Tyrosine Engineered Using Multicolor Flow Cytometry. J Am Chem Soc 131(50):18186-90. PDF
Varadarajan N, Cantor J, Georgiou G and Iverson BL (2009) Construction and flow-cytometric screening of targeted enzyme libraries. Nat Prot 4(6):893-901. PDF
Varadarajan N, Georgiou G, Iverson BL (2008) Engineered Proteases that Cleave Specifically after Sulfalted Tyrosine for the Detection of Post-Translationally Modified Peptides. Angew Chem Intl Ed 47(41):7861. PDF
Varadarajan N et al. (2008) Highly Active and Selective Endopeptidases with Programmed Substrate Specificities. Nat Chem Biol 4(5):290-4. PDF
Hwang BY et al. (2007) Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpP. J Bacteriol 189: 522-30. PDF
Varadarajan N et al. (2005) Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. Proc Natl Acad Sci U S A 102:6855-60. PDF