Single-Cell Lab

Publications

2010-Present

  • Doshi A et al. (2020) Small-molecule inducible transcriptional control in mammalian cells. Crit Rev in Biotech PDF
  • An X et al. (2020) Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2. bioRxiv PDF
  • Rath J et al. (2020) Single-cell transcriptomics identifies multiple pathways underlying antitumor function of TCR- and CD8αß -engineered human CD4+ T cells. Science Advances PDF
  • Daher M et al. (2020) CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor. bioRxiv PDF
  • Zheng J et al. (2020) Single cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia. bioRxiv PDF
  • Fousek R et al. (2020) CAR T-cells that target acute B-lineage leukemia irrespective of CD19 expression Leukemia PDF
  • Yang D et al. (2020) Mesoscopic Liquid Clusters Represent a Distinct Condensate of Mutant p53. bioRxiv PDF
  • Mobiny A et al. (2020) Automated Classification of Apoptosis in Phase Contrast Microscopy Using Capsule Network IEEE Trans Med Imaging   PDF
  • Ramesh P et al. (2019) Engineered Chymotrypsin for Mass Spectrometry-Based Detection of Protein Glycosylation. ACS Chem Biol   PDF
  • Yuan P et al. (2019) Phasetime: Deep Learning Approach to Detect Nuclei in Time Lapse Phase Images J Clin Med   PDF
  • Varadarajan N and Mohan C (2019) Elucidating the molecular circuitry of autoimmunity Nat Immunol   PDF
  • Soundarajan R et al. (2019) Targeting the Interplay between Epithelial-to-Mesenchymal-Transition and the Immune System for Effective Immunotherapy Cancers   PDF
  • Yankaskas C et al. (2019) A microfluidic assay for the quantification of the metastatic propensity of breast cancer specimens Nature Biomed Engineering   PDF
  • Mahendra A et al. (2019) Beyond Autoantibodies: Biologic Roles of Human Autoreactive B Cells in Rheumatoid Arthritis Revealed by RNA-Sequencing. Arthritis Rheumatol   PDF
  • Lu H et al. (2019) TIMING 2.0: high-throughput single-cell profiling of dynamic cell-cell interactions by time-lapse imaging microscopy in nanowell grids. Bioinformatics   PDF
  • An X, and Varadarajan N (2018) Single-cell technologies for profiling T cells to enable monitoring of immunotherapies. Curr Opin Chemical Engg   PDF
  • An X et al. (2017) Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells. Plos One   PDF
  • Ritthipichai K et al. (2017) Multifaceted role of BTLA in the control of CD8+ T cell fate after antigen encounter Clin Cancer Res   PDF
  • Varadarajan N (2017) Unmassked: Single-Cell Profiling of Immune Cell Populations in Tumors. Mol Ther   PDF
  • Lee CH et al. (2017) IgG Fc domains that bind C1q but not effector Fcg receptors delineate the importance of complement-mediated effector functions. Nat Immunol 196: 4075-4081  PDF
  • Moogk D et al. (2016) Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. J Immunol 196: 4075-4081  PDF
  • Mahendra A et al. (2016) Generation of Catalytic Antibodies Is an Intrinsic Property of an Individual’s Immune System: A Study on a Large Cohort of Renal Transplant Patients. J Immunol 196: 4075-4081.   PDF
  • Ramesh B; Frei CS; Cirino PC; Varadarajan N (2015) Functional enrichment by direct plasmid recovery after fluorescence activated cell sorting. Biotechniques 59(3), 157-161.   PDF
  • Merouane A et al. (2015) Automated and Quantitative Profiling of Individual Cell-Cell Interactions from High-throughput Time-lapse Imaging Microscopy in Nanowell Grids. (TIMING) Bioinformatics 31(19):3189-97.   PDF
  • Liadi I et al. (2015) Defining potency of CAR+ T cells: Fast and Furious or Slow and Steady. OncoImmunology doi: 10.1080/2162402X.2015.1051298   PDF
  • Liadi I et al. (2015) Individual motile CD4+ T cells can participate in efficient multi-killing through conjugation to multiple tumor cells. Cancer Immunol Res 3(5):473-82.   PDF
  • Romain G et al. (2014) Antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells. Blood Nov 20;124(22):3241-9.   PDF
  • Ogunniyi AO et al. (2014) Profiling human antibody responses by integrated single-cell analysis. Vaccine May 19;32(24):2866-73.   PDF
  • Sendra VG, Lie A, Romain G, Agarwal SK, Varadarajan N (2013) Detection and isolation of auto-reactive human antibodies from primary B cells. Methods 2013 Dec 1;64(2):153-9.   PDF
  • Dekosky BJ et al. (2013) High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nat Biotech 2013 Feb;31(2):166-9.   PDF
  • Liadi I et al. (2013) Quantitative High-Throughput Single-Cell Cytotoxicity Assay for T cells. J Vis Exp 2013 Feb 2;(72).   PDF
  • Ramesh B, Sendra VG, Cirino PC, Varadarajan N (2012) Single-cell characterization of autotransporter mediated Escherichia coli surface display of disulfide-bond containing proteins. J Biol Chem Nov 9;287(46):38580-9.   PDF
  • Skretas G et al. (2012). Multi-copy genes that enhance the yield of mammalian G Protein-coupled receptors in Escherichia coli. Metab Eng 2012 Sep;14(5):591-602.   PDF
  • Varadarajan N et al. (2012) Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc Natl Acad Sci U S A 109(10):3885-90.   PDF
  • Varadarajan N et al. (2011) A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis. J Clin Invest Nov 1;121(11):4322-31.   PDF

2005-2010

  • Varadarajan N, Pogson M, Georgiou G and Iverson BL (2009) Proteases That Can Distinguish among Different Post-translational Forms of Tyrosine Engineered Using Multicolor Flow Cytometry. J Am Chem Soc 131(50):18186-90.   PDF
  • Varadarajan N, Cantor J, Georgiou G and Iverson BL (2009) Construction and flow-cytometric screening of targeted enzyme libraries. Nat Prot 4(6):893-901.   PDF
  • Varadarajan N, Georgiou G, Iverson BL (2008) Engineered Proteases that Cleave Specifically after Sulfalted Tyrosine for the Detection of Post-Translationally Modified Peptides. Angew Chem Intl Ed 47(41):7861.   PDF
  • Varadarajan N et al. (2008) Highly Active and Selective Endopeptidases with Programmed Substrate Specificities. Nat Chem Biol 4(5):290-4.   PDF
  • Hwang BY et al. (2007) Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpP. J Bacteriol 189: 522-30.   PDF
  • Varadarajan N et al. (2005) Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. Proc Natl Acad Sci U S A 102:6855-60.   PDF
Back to top